|
KMID : 0380020060210020090
|
|
Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 2 p.90 ~ p.95
|
|
|
Enhanced Lycopene Production in Recombinant Escherichia coli by Random Transposon and NTG Mutagenesis
|
|
Yoon Sang-Hwal
Ko Min-Su
Park Kyoung-Ae
Jung Kyung-Hwa
Shin Yong-Chul
Lee Young-Mi
Lee Sook-Hee
Kim Seon-Won
|
|
|
Abstract
|
|
|
|
Escherichia coli harboring pAC-LYCO4 and pDdxs was used for lycopene production. Three wild type strains of E. coli OW1, MG1655, and W3110 were compared with DH5α used before for lycopene production. Lycopene productivity of E. coli MG1655 was similar to DH5α and the highest among those wild type strain. Therefore, MG1655 strain was used for random transposon and NTG mutagenesis to increase lycopene productivity. Through transposon mutation, five transposon mutants with increased lycopene productivity were obtained. It was found that genes knocked out by transposon insertion were treB in Tn1 mutant, B2436 in Tn2 mutant, and rfaH in Tn3, 4, and 5 mutants. Lycopene productivity was the highest in Tn4 mutant among the Tn mutants, which was 6-fold and 8-fold higher in lycopene concentration and content, respectively, in comparison with those obtained with wild type strain. NTG4 mutant was acquired with NTG mutation. The highest lycopene productivity of 6 mg/L and 4 mg/g DCW was obtained from the NTG4 mutant when arabinose of 0.013 mM was added for induction of dxs, rate-limiting gene of MEP pathway. The lycopene productivity of NTG4 mutant was increased 18-fold and 12-fold in lycopene concentration and content, respectively when comparing with the wild type strain.
|
|
|
KEYWORD
|
|
|
Lycopene, random mutation, transposon, NTG, Escherichia coli
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|